Some Nigerian males across various geographic and socioeconomic strata employ the use of herbals such as Cissus populnea extracts. Cissus populnea (Guill & Perr): A Study of the Aqueous Extract as Potential Spermatogenic Enhancers in Male Wistar Rats. Anthony B. Ojekale, Oladipupo A . Cissus populnea Guill. & Perr. [family VITACEAE]. Herbarium. Royal Botanic Gardens, Kew (K). Collection. Useful Plants of West Tropical Africa. Resource Type.

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There are quite a number of herbals presently available and commercially sold in the public domain in Nigeria which the manufacturers claim to enhance both libido and popuknea. Ascorbic acid is a good reducing agent and exhibits its antioxidant activities by electron donation [ 2526 ]. The powdered stem bark of C.

International Scholarly Research Notices

ROS can directly attack the polyunsaturated fatty acids of the cell membranes and induce lipid peroxidation. Determination of Reducing Property The reducing property of the extract was determined by assessing the ability of the extract to reduce FeCl 3 solution as described by Oyaizu [ 21 ]. Animals The procured Wistar rats were housed in well ventilated cages, kept in the University animal house and allowed to acclimatize in their new environment for 7 days.

International journal of fertility, Table 1 Thin layer chromatographic results of anthraquinone extract of Cissus populnea stem bark and Senna leaves. Is there a common cause?

Chemicals and Reagents Chemicals and reagents used such as thiobarbituric acid TBA1,phenanthroline, deoxyribose, gallic acid, and Folin-Ciocalteau’s reagent were procured from Sigma-Aldrich, Inc.

Nigerian Quarterly Journal of Hospital Medicine

Determination of Vitamin C Content Vitamin C content of the sample was determined using the method of Benderitter et al. Some Nigerian males across various geographic and socio-economic strata employ the popilnea of herbals such as Cissus populnea extracts amongst others to manage this and also as herbal aphrodisiacs.


DPPH radical scavenging ability of aqueous extract of stem bark of Cissus populnea. The stem of Cissus populnea were collected in Aprilin the morning from Tashar Ango, a village 60km along Zaria-Kano road. Preparation of Tissue Homogenates The rats were decapitated under mild diethyl ether anaesthesia, and the testes tissue were rapidly dissected and placed on ice and weighed.

Ojekale 1, Oladipupo A. The epididymis was used for estimation of sperm parameters. The ability of the water extractible phytochemicals from stem bark of C.

Lipid Peroxidation Assay 2. DPPH is a free radical donor that accepts an electron or hydrogen to become a stable diamagnetic molecule [ 42 ].

The animals were used in accordance with the procedure approved by the Animal Ethics Committee of the Federal University of Technology, Akure, Nigeria. African Journal of Biotechnology, Sperm density was determined by modifying a method previously described [ 25 ].

West African Plants – A Photo Guide – Cissus populnea Guill. & Perr.

Only a small proportion of tubules showed germ cell loss especially in the ad-luminal areas. The rats were decapitated under mild diethyl ether anaesthesia, and the testes tissue were rapidly popupnea and placed on ice and weighed. This followed a procedure as described [ 45 ].

Malondialdehyde MDA is the end-product of lipid peroxidation, which is a process where reactive oxygen species ROS degrade polyunsaturated fatty acids.

Results and Discussion References. The chromatographic analysis indicated the presence of physcion and chrysaphanol; by their corresponding colours, R f values Table 1 and with reference to the chramotographed standard senna and R f values cissue senna obtained from the literature Friedrich and Bailer, The use of C.


Briefly, the testes was cut into 0. Phenolic compounds can protect the human body from free radicals, whose formation is associated with the normal metabolism of aerobic cells.

Sample Collection Fresh samples of stem bark of Cissus populnea were purchased in a local market, in Akure metropolis, Nigeria. Previous phytochemical studies on different parts of C. Flora of West tropical Africa. Author information Copyright and License information Disclaimer. This project aims at determining the type of anthraquinones from the stem bark of C. Significance was accepted at.

Other reasons such as oxidative stress [ 16 ]weight, disease conditions, and lifestyle are some of the establisehd factors that affect libido in men. The presence of anthraquinones justifies the use of the stem bark in the treatment of indigestion and the chromatographic profile can be used for identification of the plant.

Anthraquinones of Cissus Populnea Guill & Perr (Amplidaceae)

Sertoli cells support all cells of the germ series as they pass through the various stages of development from primordial spermatogonia to fully differentiated free spermatozoa. Cissus populnea cisss, stem bark, anthraquinones, TLC. Fertility and sterility, There was also an elevation in absolute weight of the testis and prostate in both C.

Izzo, The aphrodisiac and adaptogenic properties of ginseng. Nigerian Quarterly Journal of Hospital Medicine, American Journal of Medical and Biological Research3 5 Evidence has shown that these damaging events are caused by free radicals [ 1 ].

Data were analysed with SPSS Popupnea Infertility is a major clinical problem, affecting people medically and psychosocially.